Next Generation Sequencing / Lab on A Chip / DNA Synthesis

24. March 2010 by admin.

I recently contacted Bryan Bishop of the H+ community in regards to what the state of the art of DIY synthesis was, since I was interested in seeing if we can make an inexpensive unit. He suggested that the inkjet synthesis was far too bulky, and suggested I check out the newer Lab on a Chip/microfluidic assemblies. What many array manufacturers are referring to as NGS or Next Generation Sequencing, that can put together entire chromosomes.

Here are a few of the players in the field as of the moment.

Bryan has a whole bunch of DIY-based information on the subject, and  is willing to  correspond. Unfortunately most of this is abit over my head with my current lack of engineering background. I’m hoping a few of you, might have some interest in following this up.

“Right now there’s a lot of background community support for building a DIY DMD micromirror array for photolabile-based DNA synthesis. These rrays cost about $300 for 1024×2048 cells. The major cost otherwise
is documenting, confirming and purchasing the requisite chemicals. The inkjet plotter approach is interesting, but big and bulky and very expensive. Hell, PoSaM was in a nitrogen atmosphere or something. So, if you’re interested in the DIY DMD micromirror array approach, please it me up.. I have lots of literature that you can download on the subject.

And in general I have lots of literature available here:
http://designfiles.org/papers/
http://designfiles.org/papers/microfluidics/
.. and if you do express an interest in the DMD approach, I’ll dig up the relevant papers.”

Thanks Bryan, we’ll definately be getting back in contact with you regarding this.

Posted in Networking, DIY, Synthetix, Academic | Print | No Comments »